Cytosolic beta-glucosidase, also known as cytosolic beta-glucosidase-like protein 1, is a beta-glucosidase () enzyme that in humans is encoded by the GBA3 gene.
Cytosolic beta-glucosidase is a predominantly liver enzyme that efficiently hydrolyzes beta-D-glucoside and beta-D-galactoside, but not any known physiologic beta-glycoside, suggesting that it may be involved in detoxification of plant glycosides. GBA3 also has significant neutral glycosylceramidase activity (), suggesting that it may be involved in a non-lysosomal catabolic pathway of glucosylceramide metabolism.