Estiaiotida () is a former municipality in the Trikala regional unit, Thessaly, Greece. Since the 2011 local government reform, it is part of the municipality Trikala, of which it is a municipal unit. The municipal unit has an area of 39.762 km<sup>2</sup>. Population 2,452 (2021). The seat of the municipality was in Megalochori. The municipality was named after Histiaeotis, an ancient district of Thessaly region.