The Roman Catholic Diocese of La Guaira () is a diocese located in the city of La Guaira in the ecclesiastical province of Caracas in Venezuela.
On 15 April 1970 Pope Paul VI established the Diocese of La Guaira from the Metropolitan Archdiocese of Caracas.
La Guaira is home to the oldest pilgrimage to Our Lady of Lourdes in Latin America. The devotion began in 1884 when a Venezuelan priest, Fr Machado, returned from a pilgrimage to the original Lourdes grotto in France. He established a shrine in the mountain of El ÃÂvila. Every year, with only very few exceptions, a 20 Km pilgrimage takes place along the Camiño de los Españoles, beginning in Puerto Caracas and ending in the church of St Sebastian, in MaiquetÃÂa.