Dimethylglycine dehydrogenase () is an enzyme that catalyzes the chemical reaction
The three substrates of this enzyme are dimethylglycine, water and an electron-transferring flavoprotein. Its products are sarcosine, formaldehyde, and the reduced flavoprotein.
This enzyme belongs to the family of oxidoreductases, specifically those acting on the CH-NH group of donors with other acceptors. The systematic name of this enzyme class is N,N-dimethylglycine:acceptor oxidoreductase (demethylating). Other names in common use include N,N-dimethylglycine oxidase, and N,N-dimethylglycine:(acceptor) oxidoreductase (demethylating). This enzyme participates in glycine and threonine metabolism. It employs one cofactor, flavin adenine dinucleotide.