Daulatpur () is an upazila of Kushtia District in the Division of Khulna, Bangladesh. Daulatpur Thana was formed in 1854. It became an upazila in 1983.
Daulatpur is located at . It has 115,715 households and total area 468.76 km<sup>2</sup>.
Daulatpur Upazila is bounded by Bagha Upazila in Rajshahi District and Lalpur Upazila in Natore District, on the north, Bheramara and Mirpur Upazilas on the east, Gangni Upazila in Meherpur District and Mirpur Upazila in Kushtia District, on the south, and Jalangi CD Block, in Murshidabad district, West Bengal, India, and Karimpur I and Karimpur II CD Blocks, Nadia district, West Bengal, on the west.
According to the 2022 Bangladeshi census, Daulatpur Upazila had 132,529 households and a population of 482,981. 8.65% of the population were under 5 years of age. Daulatpur had a literacy rate (age 7 and over) of 64.88%: 66.18% for males and 63.67% for females, and a sex ratio of 95.02 males for every 100 females. 36,842 (7.63%) lived in urban areas.
Daulatpur Upazila is divided into 14 union parishads: Adabaria, Ariaa, Boalia, Chilmari, Daulatpur, Hogalbaria, Khalishakundi, Maricha, Mathurapur, Pearpur, Philipnagar, Pragpur, Ramkrishnapur, and Refaitpur. The union parishads are subdivided into 82 mauzas and 103 villages.