TRNA (cytosine38-C5)-methyltransferase (, hDNMT2 (gene), DNMT2 (gene), TRDMT1 (gene)) is an enzyme with the systematic name S-adenosyl-L-methionine:tRNA (cytosine38-C5)-methyltransferase. This enzyme catalyses the following chemical reaction:
The eukaryotic enzyme catalyses methylation of cytosine<sup>38</sup> in the anti-codon loop of tRNAAsp(GTC), tRNAVal(AAC) and tRNAGly(GCC).