In enzymology, D-glutamate(D-aspartate) oxidase () is an enzyme that catalyzes the chemical reaction
The three substrates of this enzyme are D-glutamic acid, water, and oxygen. Its products are ñ-ketoglutaric acid, hydrogen peroxide, and ammonia. The enzyme can also act in a similar way on D-aspartic acid.
This enzyme belongs to the family of oxidoreductases, specifically those acting on the CH-NH<sub>2</sub> group of donors with oxygen as acceptor. The systematic name of this enzyme class is D-glutamate(D-aspartate):oxygen oxidoreductase (deaminating). Other names in common use include D-glutamic-aspartic oxidase, and D-monoaminodicarboxylic acid oxidase. This enzyme participates in alanine and aspartate metabolism. It employs one cofactor, flavin adenine dinucleotide.