This is a list of every player to have been listed in the Australian Football League or the AFL Women's for the Brisbane Lions in the club's history.
Players are listed in order of debut, and the start of their Brisbane Lions career is determined by their year of debut, and the end is determined by the year of their final game playing for the Brisbane Lions. This guideline results in the possibility that players may not have necessarily begun or finished their career on the Brisbane Lions' list within these periods listed.
For the 2022 season, clubs were permitted a supplementary list of 20 state league players, who would be available to play in the AFL if players were unavailable due to the league's health and safety protocols during the COVID-19 pandemic. Brisbane selected VFL players from Coburg, Port Melbourne and their own reserves team. None played an AFL game for the Lions in 2022, though Bruce Reville later played for the club at AFL level.