Chignolo d'Isola (; ) is a (municipality) in the Province of Bergamo in the Italian region of Lombardy, located about northeast of Milan and about southwest of Bergamo. As of 31 December 2004, it had a population of 2,849 and an area of .
Chignolo d'Isola borders the following municipalities: Bonate Sopra, Bonate Sotto, Bottanuco, Madone, Medolago, Suisio, Terno d'Isola.
In the territory of Chignolo d'Isola on 26 February 2010 the body of Yara Gambirasio was found.