Cherkasy Regiment () was a military unit and administrative subdivision of the Cossack Hetmanate centered in the city of Cherkasy. It existed between 1625 and 1686. After the 1667 Treaty of Andrusovo the regiment was incorporated by the Kingdom of Poland.
The regiment was created in 1625 in the territories of Cherkasy starostvo. Its establishment took place according to the terms of Treaty of Kurukove. The regiment's formation was confirmed by the 1649 Treaty of Zboriv. In 1678-1679 the regiment's territory was devastated by troops of Moscow-allied hetman Ivan Samoilovych, who forcibly resettled the local population to Left-bank Ukraine, razing Cherkasy and other cities in the area.
According to the 1649 Registry of the Zaporozhian Host, the regiment included following geographical subdivisions: