Charlois () is a neighbourhood of the Dutch city of Rotterdam. It is located on the south bank of the Nieuwe Maas.
Charlois used to be a separate municipality until 1895, when it became part of Rotterdam.
Charlois used to have an airport. Waalhaven airport as it was called, was opened in 1920 as the second civilian airport in the Netherlands after Schiphol. The airport was destroyed in 1940 by the Dutch army so it could not be used by invading German Wehrmacht during World War II.