Cane Creek Canyon Nature Preserve is a private nature preserve in Colbert County, Alabama, south of Tuscumbia that opened in 1996 and is owned by The Land Trust of North Alabama (previously and historically owned by Dr. Jim and Faye Lacefield). The couple purchased of land in 1979 and gradually added land, growing to over . The land's features include waterfalls, wetlands, streams, glades, cliffs, and the canyon. The preserve, which is open to the public, has of hiking trails, and access to the preserve is free.
As of 2025, the preserve contains 25.7 miles (41.4 km) of marked hiking trails of varying difficulty, ranging from easy to strenuous. The preserve is open to visitors year-round, Friday through Sunday, from 7:00 am to 5:00 pm, including all holidays except Thanksgiving and Christmas. Visitors are required to sign in at the trailhead kiosk before beginning their hike. The preserve remains free to access and is operated by the Land Trust of North Alabama, which assumed ownership in 2023 and continues ongoing trail development, habitat management, and public access.