Byssotrema is a monotypic fungal genus in the subfamily Graphidoideae of the family Graphidaceae. It contains the single species Byssotrema mirabile, a little-known corticolous (bark-dwelling), crustose lichen found only in Brazil.
Both the genus Byssotrema and its single species were described as new to science in 2014 by the lichenologists Marcela Cáceres, André Aptroot, and Robert Lücking. The type specimen of Byssotrema mirabile was collected in from the Cuniã Ecological Station (Rondônia), at kilometer 760 on road BR 319, northwest of Porto Velho, at an elevation of . The specimen was found growing on tree bark in a primary rainforest on 13 March 2012 by Cáceres and Aptroot.
The genus name Byssotrema is derived from the Greek , meaning "fine and soft fabric". The ending âÂÂtrema is derived from the Greek neuter noun , meaning "perforation; aperture; opening; orifice", and refers to the widely open ascomata that are characteristic of the genus.
The thallus of Byssotrema mirabile can reach up to about in diameter and has a continuous, smooth surface with a greenish-grey colour. The thallus lacks a (a distinct boundary layer). In cross-section, the thallus is 70âÂÂ100 üm thick and comprises a cortex (a type of cellular structure) 15âÂÂ20 üm thick, a photobiont layer 30âÂÂ40 üm thick, and a medulla 30âÂÂ40 üm thick. The medulla contains numerous small, grey crystals that do not dissolve in potassium hydroxide solution. The , or photosynthetic partner, is from the green algal genus Trentepohlia, with cells that are rounded to irregular in shape, arranged in irregular groups, and yellowish-green in colour, measuring 7âÂÂ11 by 6âÂÂ9 üm.
The ascomata (fruiting bodies) are rounded to angular, prominent to , and measure 0.5âÂÂ1 mm in diameter and 0.4âÂÂ0.5 mm in height. They feature a nearly complete, steeply sloping that is smooth and greyish-green, with a distinct that appears as a thick, white rim around the pore. The of the ascomata is partially exposed, light brown, translucent, and thinly white- (having a frost-like appearance).
The (the outer layer of the ascomata) is apically , with orange-brown lateral and basal portions, 30âÂÂ50 üm wide. It is covered laterally by an algiferous (algae-bearing), corticate thallus that lacks periderm layers and is 60âÂÂ90 üm thick. The (the layer below the hymenium) is prosoplectenchymatous, 10âÂÂ15 üm high, and hyaline to yellowish. The hymenium (the spore-bearing layer) is 90âÂÂ100 üm high, hyaline, and strongly (infused with granules), which rapidly dissolve in potassium hydroxide. The epithecium (the uppermost layer of the hymenium) is indistinct, 5âÂÂ10 üm high, and hyaline.
The paraphyses (sterile filaments in the hymenium) are unbranched and smooth at the tips. The asci (spore sacs) are to , measuring 80âÂÂ90 by 10âÂÂ12 üm. Each ascus contains 8 ellipsoid ascospores, which are 3-septate, measuring 11âÂÂ15 by 6âÂÂ7 üm, and 1.8âÂÂ2.5 times as long as wide. The ascospores are hyaline, with lens-shaped lumina, and react violet-blue with iodine.
Byssotrema mirabile is exclusively found in the primary rainforests of Rondônia, Brazil, where it grows in the shaded understory of rainforest environments undisturbed by man. It is only known to occur at its type locality.