Buckhorn is an unincorporated community in southeastern Madison County, Missouri, United States.
The community is located on Missouri Route M at the location where the West, Middle and East forks of Big Creek converge. The site is approximately north of the Madison-Wayne county line. The community of Cascade is about south on Big Creek.
A post office called Buckhorn was established in 1901, and remained in operation until 1959. The community took its name from a nearby creek of the same name where bucks were abundant.