Bratslav Regiment () was an administrative division of the Cossack Hetmanate which existed between 1648 and 1712. It was located in southern Podolia. The regiment's administrative capital was Bratslav.
Creation of the regiment was officially recognized by the Treaty of Zboriv in 1649. In 1654 Cossacks of Bratslav Regiment refused to pledge allegiance to tsar Alexis of Russia following the Treaty of Pereyaslav. During 1674-1675 most of the regiment's population fled due to fighting between the Polish troops of Jan Sobieski and Ottoman armies allied with Right-bank hetman Petro Doroshenko.
In 1675 the regiment's Cossacks recognized Polish appointee Ostap Hohol as acting hetman. In 1685 the Sejm confirmed the rights and privileges of local Cossacks, which led to the revival of Bratslav Regiment. The regiment was liquidated following the Treaty of the Pruth, and its territory was reincorporated into the Polish-Lithuanian Commonwealth.
According to the 1649 Register of the Zaporozhian Host, the regiment consisted of following sotnias: