Bolsheglushitsky District () is an administrative and municipal district (raion), one of the twenty-seven in Samara Oblast, Russia. It is located in the south of the oblast. The area of the district is . Its administrative center is the rural locality (a selo) of Bolshaya Glushitsa. Population: 20,477 (2010 Census); The population of Bolshaya Glushitsa accounts for 47.2% of the district's total population.