my-server
← Wiki

Bismuth sulfite agar

Bismuth sulfite agar is a type of agar media used to isolate Salmonella species. It uses glucose as a primary source of carbon. Bismuth and brilliant green (dye) both inhibit gram-positive growth. Bismuth sulfite agar tests the ability to use ferrous sulfate and convert it to hydrogen sulfide.

Bismuth sulfite agar typically contains (w/v):

:1.6% bismuth sulfite Bi<sub>2</sub>(SO<sub>3</sub>)<sub>3</sub>
:1.0% pancreatic digest of casein
:1.0% pancreatic digest of animal tissue
:1.0% beef extract
:1.0% glucose
:0.8% dibasic sodium phosphate
:0.06% ferrous sulfate • 7 water
:pH adjusted to 7.7 at 25&nbsp;°C

This medium is boiled for sterility, not autoclaved.

References