Bethlehem College was an Independent Roman Catholic comprehensive single-sex secondary day school for girls, located in Bland Street, Ashfield, the Inner West of Sydney, New South Wales, Australia. The college was founded in 1881 by the Sisters of Charity. In 2022, the school announced plans to amalgamate with the adjacent boys' high school, De La Salle College Ashfield, and St Vincent's Primary School, due to increasing demand for co-educational schools in inner Sydney. Since 2023, the former campus does not exist as an independent school but remains a campus of the co-eduational college St Vincent's College Ashfield, that was formed in the amalgamation.
The college was founded in 1881. In the early twentieth century one of the college's Principal was Dorothy Josephine Bruton who was known as Sister Dympna.