Barnes Creek is a stream in Wayne County in the U.S. state of Missouri. It is a tributary of Bear Creek.
The stream headwaters arise in northeastern Wayne County north of Missouri Route P (at ) and the stream flows generally north to its confluence with Bear Creek just northeast of the community of Lowndes adjacent to Missouri Route E (at ).
Barnes Creek, historically called "Barnes Fork", has the name of Clayburn Barnes, the original owner of the site.