Austrofundulus is a genus of killifish in the family Rivulidae native to northern Colombia, northern Venezuela and southwestern Guyana. They are annual killifish where adults generally have a short life in temporary ponds or swamps and the eggs experience a period of drought, only hatching when again covered by water.
They are small thickset fish, no more than in total length, and most less than half that size.
Austrofundulus is closely related to Rachovia, and it has been suggested that the former should be merged into the latter.
There are currently 7 recognized species of Austrofundulus: