Australothele nothofagi is a species of mygalomorph spider in the Euagridae family. It is endemic to Australia. It was described in 1984 by Australian arachnologist Robert Raven.
The species occurs in the border ranges of south-eastern Queensland and north-eastern New South Wales in closed forest habitats. The type locality is Lamington National Park.
The spiders are arboreal and terrestrial predators which construct tubular silk shelters in the ground and in the bark of Nothofagus butts.