The Apostolic Vicariate (or Vicariate Apostolic) of Tucupita () is a missionary circonscription of the Roman Catholic Church in Venezuela.
It is exempt, i.e. directly dependent on the Holy See, not part of any ecclesiastical province. Its cathedral see is located in Tucupita, the city capital of Venezuela's Atlantic-coastal Delta Amacuro state.
On 30 July 1954, Pope Pius XII established the Apostolic Vicariate of Tucupita from territory taken from the Apostolic Vicariate of CaronÃÂ.
Note: so far, all its Apostolic Vicars were Capuchins and titular bishops