The Lake Afdera killifish (Aphaniops stiassnyae) is a species of fish in the family Aphaniidae. It is found in Lake Afdera in Ethiopia. The species was evaluated by the IUCN on 1 May 2009 and listed as endangered on the Red List.
The species name, stiassnyae, is named after Melanie L.J. Stiassny, who studied African fish.
The Lake Afdera killifish reaches a maximum length of . It has a sharply upturned lower jaw; it is also one of two species in its genus to possess conical teeth.