Anna of Saxe-Wittenberg (died 22 November 1327) was a German noblewoman of the House of Ascania. By her marriage to Henry II, Lord of Mecklenburg, she served as the Lady of Mecklenburg during a period of significant territorial expansion for the House of Mecklenburg.
Anna was the daughter of Albert II, Duke of Saxony and Agnes of Austria, a daughter of King Rudolf I of Germany. Her lineage connected the Saxon ducal house with the rising House of Habsburg, which significantly enhanced the political prestige of her husband's court.
In 1308, Anna married Henry II, Lord of Mecklenburg as his second wife..The union produced the following children:
Anna died in November 1327. Following her death, Henry II contracted a third marriage with Anna of Brandenburg-Stendal to further consolidate political ties between the Ascanian and Mecklenburg houses.