Ancylotrypa coloniae, commonly known as the Jansenville wafer-lid trapdoor spider, is a species of spider of the genus Ancylotrypa. It is endemic to the Eastern Cape, South Africa.
Ancylotrypa coloniae is an Eastern Cape endemic known only from the type locality of Jansenville, at an elevation of 444 meters above sea level.
Free-living ground dwellers that live in silk-lined burrows. The species has been sampled from the Thicket biome.
Only the female has been described for this species.
Listed as Data Deficient on the South African Red List for taxonomic reasons. More sampling is needed to collect the male and determine the species' range.