The enzyme amygdalin ò-glucosidase () catalyzes the following chemical reaction:
Thus, the two substrates of this enzyme are (R)-amygdalin and H<sub>2</sub>O, whereas its two products are (R)-prunasin and <small>D</small>-glucose.
This enzyme belongs to the family of hydrolases, specifically those glycosidases that hydrolyse O- and S-glycosyl compounds. The systematic name of this enzyme class is amygdalin beta-<small>D</small>-glucohydrolase. Other names in common use include amygdalase, amygdalinase, amygdalin hydrolase, and amygdalin glucosidase.
It can be completely inhibited by the action of Glucono-ô-lactone at 1 mM concentration.