Amphylaeus is a genus of plasterer bee in the Colletidae family and Hylaeinae subfamily. The genus is endemic to Australia.
Amphylaeus bees are slender, less than 10 mm long, and black with pale, mask-like facial markings. Some have a yellow spot on the thorax. They nest in pithy stems or preexisting holes in wood. Their brood cells are woven from a cellophane-like secretion.
Amphylaeus contains the following subgenera:
Species include: