Ambrosio Fracassini, O.P. (died 1663) was a Roman Catholic prelate who served as Bishop of Pula (1663).
Ambrosio Fracassini was born in Brescia, Italy on 8 Dec 1597 and ordained a priest in the Order of Preachers. On 12 Mar 1663, he was appointed by Pope Alexander VII as Bishop of Pula. On 27 Mar 1663, he was consecrated bishop by Scipione Pannocchieschi d'Elci, Archbishop of Pisa. He served as Bishop of Pula until his death on 22 Sep 1663.