Ambernath Assembly constituency is one of the 288 Vidhan Sabha (legislative assembly) constituencies of Maharashtra state, western India. This constituency is located in Thane district.
The constituency comprises parts of Ambernath taluka viz. revenue circles Ambernath (Rural), and Ambernath Municipal Council, parts of Ulhasnagar taluka that is parts of Ulhasnagar Municipal Corporation that is wards 14 to 22 and 44 to 51.