TRNA (carboxymethyluridine<sup>34</sup>-5-O)-methyltransferase (, ALKBH8, ABH8, Trm9, tRNA methyltransferase 9) is an enzyme with systematic name S-adenosyl-L-methionine:tRNA (carboxymethyluridine<sup>34</sup>-5-O)-methyltransferase. This enzyme catalyses the following chemical reaction
The enzyme catalyses the posttranslational modification of uridine residues at the wobble position 34 of the anticodon loop of tRNA.