ACV synthetase (ACVS, <small>L</small>-ô-(ñ-aminoadipoyl)-<small>L</small>-cysteinyl-<small>D</small>-valine synthetase, N-(5-amino-5-carboxypentanoyl)-<small>L</small>-cysteinyl-<small>D</small>-valine synthase, ) is an enzyme that catalyzes the chemical reaction
The five substrates of this enzyme are ATP, <small>L</small>-2-aminohexanedioate, <small>L</small>-cysteine, <small>L</small>-valine, and H<sub>2</sub>O, whereas its three products are AMP, diphosphate, and N-[<small>L</small>-5-amino-5-carboxypentanoyl]-<small>L</small>-cysteinyl-<small>D</small>-valine.
ACVS is an example of a nonribosomal peptide synthetase (NRPS). It participates in penicillin and cephalosporin biosyntheses.