In enzymology, a 4-methyleneglutamateâÂÂammonia ligase () is an enzyme that catalyzes the chemical reaction
The 3 substrates of this enzyme are ATP, 4-methylene-L-glutamate, and NH<sub>3</sub>, whereas its 3 products are AMP, diphosphate, and 4-methylene-L-glutamine.
This enzyme belongs to the family of ligases, specifically those forming carbon-nitrogen bonds as acid-D-ammonia (or amine) ligases (amide synthases). The systematic name of this enzyme class is 4-methylene-L-glutamate:ammonia ligase (AMP-forming). This enzyme is also called 4-methyleneglutamine synthetase. This enzyme participates in c5-branched dibasic acid metabolism.