In enzymology, a 4-chlorobenzoate dehalogenase () is an enzyme that catalyzes the chemical reaction
Thus, the two substrates of this enzyme are 4-chlorobenzoate and H<sub>2</sub>O, whereas its two products are 4-hydroxybenzoate and chloride.
This enzyme belongs to the family of hydrolases, specifically those acting on halide bonds in carbon-halide compounds. The systematic name of this enzyme class is 4-chlorobenzoate chlorohydrolase. This enzyme is also called halobenzoate dehalogenase.
As of late 2007, 3 structures have been solved for this class of enzymes, with PDB accession codes , , and .