In enzymology, a 4-acetamidobutyryl-CoA deacetylase () is an enzyme that catalyzes the chemical reaction
Thus, the two substrates of this enzyme are 4-acetamidobutanoyl-CoA and H<sub>2</sub>O, whereas its two products are acetate and 4-aminobutanoyl-CoA.
This enzyme belongs to the family of hydrolases, those acting on carbon-nitrogen bonds other than peptide bonds, specifically in linear amides. The systematic name of this enzyme class is 4-acetamidobutanoyl-CoA amidohydrolase. Other names in common use include aminobutyryl-CoA thiolesterase, and deacetylase-thiolesterase.