4-alpha-D-<nowiki/>{(1->4)-alpha-D-glucano}trehalose trehalohydrolase (, malto-oligosyltrehalose trehalohydrolase) is an enzyme with systematic name 4-alpha-D-((1->4)-alpha-D-glucano)trehalose glucanohydrolase (trehalose-producing). This enzyme catalyses the following chemical reaction