In enzymology, a 3alpha-hydroxycholanate dehydrogenase () is an enzyme that catalyzes the chemical reaction
The two substrates of this enzyme are the bile acid lithocholic acid and nicotinamide adenine dinucleotide (NAD<sup>+</sup>). Its products are dehydrolithocholic acid, NADH, and a proton.
This enzyme belongs to the family of oxidoreductases, specifically those acting on the CH-OH group of donor with NAD<sup>+</sup> or NADP<sup>+</sup> as acceptor. The systematic name of this enzyme class is 3alpha-hydroxy-5beta-cholanate:NAD<sup>+</sup> oxidoreductase. This enzyme is also called alpha-hydroxy-cholanate dehydrogenase.
As of late 2007, only one structure has been solved for this class of enzymes, with the PDB accession code .