The 2nd Tennessee Cavalry Regiment was a cavalry regiment that served in the Union Army during the American Civil War. It was also known as 2nd East Tennessee Cavalry.
The 2nd Tennessee Cavalry was organized July through November 1862 in eastern Tennessee and mustered in for a three year enlistment under the command of Colonel Daniel M. Ray. Subordinate officers included Lieutenant Colonel William R. Cook, and majors George W. Hutsell, Charles Inman, William R. Macbeth, and William F. Prosser. The unit was composed primarily of Southern unionists from the Tennessee counties of Knox, Sevier and Blount. Notably, among the enlisted were two women pretending to be men: Frances Elizabeth Quinn and Sarah Bradbury. The regiment's original muster rolls were destroyed at Nolensville, Tennessee on December 30, 1862. The regiment re-mustered at Murfreesboro, Tennessee on January 26, 1863.
The regiment lost a total of 224 men during service; 2 officers and 14 enlisted men killed or mortally wounded, 208 enlisted men died of disease or accident.