The 25th Bangladesh National Film Awards were presented by the Ministry of Information, Bangladesh, to felicitate the best of Bangladeshi cinema released in the year 2000. The National Film Awards are the only film awards given by the government itself. Every year, a national panel appointed by the government selects the winning entry, and the award ceremony is held in Dhaka. The ceremony took place at Osmany Memorial Hall, Dhaka, and awards were distributed by Prime Minister Khaleda Zia. Additionally, Information Minister Tariqul Islam attended the function as the special guest that evening.
A 12-member jury board headed by former secretary A H Mofazzal Karim recommended a total of 16 artists to be awarded for 2000. No awards were given in the Best Music Director or Best Lyrics categories in 2000.