The 2025 Youngstown State Penguins football team represented Youngstown State University as a member of the Missouri Valley Football Conference (MVFC) during the 2025 NCAA Division I FCS football season. The Penguins were led by sixth-year head coach Doug Phillips, and played their games at Stambaugh Stadium located in Youngstown, Ohio.
The Missouri Valley Football Conference released its preseason poll on July 21, 2025, voted on by league athletic directors, coaches, and media members. The Penguins were predicted to finish fifth in the conference.
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