The 2025 Wuxi Open was a professional tennis tournament played on hard courts. It was the second edition of the tournament which was part of the 2025 ATP Challenger Tour. It took place in Wuxi, China between 5 and 11 May 2025.
The following players received wildcards into the singles main draw:
The following player received entry into the singles main draw using a protected ranking:
The following players received entry from the qualifying draw: