The 2025 Uruguay Open was a professional tennis tournament played on red clay courts in Montevideo. It was the 20th edition of the tournament which was part of the 2025 ATP Challenger Tour. It took place at the Carrasco Lawn Tennis Club in Montevideo, Uruguay between 10 and 16 November 2025.
The following players received wildcards into the singles main draw:
The following player received entry into the singles main draw using a protected ranking:
The following player received entry into the singles main draw through the Next Gen Accelerator programme:
The following player received entry into the singles main draw as an alternate:
The following players received entry from the qualifying draw: