The 2025 Southern Miss Golden Eagles football team represented the University of Southern Mississippi in the Sun Belt Conference's West Division during the 2025 NCAA Division I FBS football season. The Golden Eagles were led by first-year head coach Charles Huff. The Golden Eagles played home games at M. M. Roberts Stadium, located in Hattiesburg, Mississippi.
The Southern Miss Golden Eagles drew an average home attendance of 22,051, the 79th-highest of all NCAA Division I FBS football teams.
In the Sun Belt preseason coaches' poll, the Golden Eagles were picked to finish fifth place in the West division.
Quarterback Braylon Braxton was named the Preseason Offensive Player of the Year and was named in the first team offense, along with defensive lineman Brodarius Lewis, defensive back Josh Moten and return specialist Ian Foster in the first team defense and special teams, respectively. Tight end Kyirin Heath was named in the second team offense.