The 2025 Southern Illinois Salukis football team represented Southern Illinois University Carbondale as a member of the Missouri Valley Football Conference (MVFC) during the 2025 NCAA Division I FCS football season. The Salukis were led by tenth-year head coach Nick Hill, and played their home games at Saluki Stadium in Carbondale, Illinois.
The Missouri Valley Football Conference released its preseason poll on July 21, 2025, voted on by league athletic directors, coaches, and media members. The Salukis were predicted to finish sixth in the conference.