The 2025 Palermo Ladies Open was a professional women's tennis tournament played on outdoor clay courts at the Country Time Club. It was the 33rd edition of the tournament and part of the 2025 WTA 125 tournaments (downgraded from WTA 250 status in previous years). It took place in Palermo, Italy, between 21 and 27 July 2025.
The following players received wildcards into the main draw:
The following player received entry using a protected ranking:
The following players received entry from the qualifying draw:
The following players received entry as lucky losers: