The 2025 UAMS Health Little Rock Open was a professional tennis tournament played on hardcourts. It was the sixth edition of the tournament which was part of the 2025 ATP Challenger Tour. It took place in Little Rock, Arkansas, United States from May 26 to June 1, 2025.
The following players received wildcards into the singles main draw:
The following player received entry into the singles main draw using a protected ranking:
The following player received entry into the singles main draw through the Junior Accelerator programme:
The following players received entry from the qualifying draw:
The following player received entry as a lucky loser: