The 2025 Liga de Ascenso Femenina season, was an amateur women's football championship, developed, organized, and promoted by the Peruvian Football Federation (FPF), which granted two direct promotion spots to the 2026 Liga Femenina. This was the 3th edition of the Liga de Ascenso Femenina and for the fourth time it had a second division character, granting direct promotion to the Liga Femenina.
Deportivo Yanapuma from Iquitos were crowned national champions and earned promotion to the 2026 Liga Femenina, becoming the first team from Loreto to reach this level.
The round was played between 18 and 19 October, in a single knock-out match format.
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The round was played between 1 and 2 November, in a single knock-out match format.
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The round was played between 15 and 16 November, in a single knock-out match format.
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