The 2025 Lexington Open was a professional tennis tournament played on outdoor hard courts. It was the 30th edition of the tournament for men, which was part of the 2025 ATP Challenger Tour, and the 28th edition for women, which was part of the 2025 ITF Women's World Tennis Tour. It took place in Lexington, Kentucky, United States, between 28 July and 3 August 2025.
The following players received a wildcard into the singles main draw:
The following player received entry into the singles main draw using a protected ranking:
The following player received entry into the singles main draw as a special exempt:
The following player received entry into the singles main draw through the College Accelerator programme:
The following player received entry into the singles main draw through the Junior Accelerator programme:
The following player received entry into the singles main draw through the Next Gen Accelerator programme:
The following player received entry into the singles main draw as an alternate:
The following players received entry from the qualifying draw:
The following players received a wildcard into the singles main draw:
The following player received entry into the singles main draw using a special ranking:
The following players received entry from the qualifying draw:
The following player received entry as a lucky loser: