The 2025 Japanese Regional Football Champions League () was the 49th edition of the referred annually contested cup for the best-placed teams of each of their respective Regional Leagues, and of the 2025 Shakaijin Cup. Due to the winner being promoted to the Japan Football League (JFL), the club couldn't defend their title. were the title holders, as they won the championship in the final round. As Asuka were in the JFL, they were unable to defend their title.
Twelve teams participated in the transitional tournament for regional league teams that want to join the JFL. Nine qualified as champions of their respective Regional Leagues in 2025. The other three spots were by default allocated to the best three Shakaijin Cup teams out of its semi-finalists that were not yet qualified to the Champions League via the Regional Leagues (being placed below the champions of their league).
In case the slots couldn't be filled, in a situation where more than one semi-finalist had already qualified to the Champions League as regional champions, the empty slot(s) was rewarded to the J.League 100 Year Plan club status holders that finished the season as a Regional League runner-up. If more than one "status holders" finished the season as the 2nd-placed team in their respective Regional Leagues, an annually-rotated order of priority is taken into consideration. Only teams that want to join the JFL can be promoted. If a team with no intention to join the JFL ends up winning the final round, the promotion spot is allocated to the runner-up. If the runner-up also has no intention to be promoted, a spot is also allocated to the third-placed team. If they also refuse, the bottom-placed team is promoted instead. In all mentioned scenarios, the bottom-placed team of the 2025 Japan Football League could be relegated, and no play-off held. If both Regional Champions League winner and runner-up aim to be promoted, the winner is automatically promoted, whereas the runner-up holds a play-off match with JFL's bottom-placed team.
Depending on the clubs that ended up as the top three teams in the Shakaijin Cup, a specific rank that would take the J.League 100 Year Plan club status clubs and its performances in the 2025 Regional Leagues into account was applied to determine the final teams in this competition.
Based on the tournament guidelines announced by the JFA
Returning teams from the previous season were noted in bold. Teams relegated from the previous JFL season were noted in italics.
First round
Final round
Each group had its matches played in a round-robin format. Each team played three matches in just three days, from 7 to 9 November, with one being played on each day.
<onlyinclude></onlyinclude>
<onlyinclude></onlyinclude>
<onlyinclude></onlyinclude>
The three winners of each group of the first round qualified for the final round, alongside the best-placed team among the runners-up of each group.
<onlyinclude></onlyinclude>
The group had its matches played in a round-robin format, held in a five-days span, from 20 to 24 November.
<onlyinclude></onlyinclude>