The 2025 James Madison Dukes football team represented James Madison University in the Sun Belt Conference's East Division during the 2025 NCAA Division I FBS football season. The Dukes were led by Bob Chesney in his second year as the head coach. The Dukes played their home games at the Bridgeforth Stadium, located in Harrisonburg, Virginia. They appeared in the College Football Playoff for the first time in program and Sun Belt history.
James Madison became the first ever program to participate in both the College Football Playoff and the FCS playoffs in the same decade (2020s), the last appearance of the latter being in 2021, which was their final season in the FCS.
The James Madison Dukes drew an average home attendance of 25,135, the 85th-highest of all NCAA Division I FBS football teams.
In the Sun Belt preseason coaches' poll, the Dukes were picked to finish first place in the East division.
Running back George Pettaway, offensive lineman Pat McMurtrie and defensive back Jacob Thomas were awarded to be in the preseason All-Sun Belt first team offense and defense, respectively. Quarterback Alonza Barnett III, defensive lineman Immanuel Bush, linebacker Trent Hendrick, defensive back DJ Barksdale and associated press player George Pettaway were named to the second team.