The 2025 Jacksonville State Gamecocks football team represented Jacksonville State University in Conference USA (CUSA) during the 2025 NCAA Division I FBS football season. The Gamecocks were led by Charles Kelly in his first year as the head coach. The Gamecocks played their home games at AmFirst Stadium, located in Jacksonville, Alabama.
The Jacksonville State Gamecocks drew an average home attendance of 19,993, the 97th-highest of all NCAA Division I FBS football teams.